Tissue Culture MCQ Questions and Answers
1. In plant tissue culture, cytokinins mainly promote the formation of which type of growth?
A. Shoot growth
B. Root growth
C. Seed dormancy
D. Leaf fall
Correct Answer:
Shoot growth
Explanation:
Cytokinins promote cell division and are commonly used in tissue culture to encourage the formation and development of shoots.
2. A high cytokinin-to-auxin ratio in a tissue culture medium generally favors the formation of:
A. Roots
B. Shoots
C. Seeds
D. Pollen grains
Correct Answer:
Shoots
Explanation:
In tissue culture, a relatively high concentration of cytokinin compared with auxin usually promotes shoot formation.
3. A plant cell grown on a suitable nutrient medium in tissue culture can develop into a complete plant because of its:
A. Totipotency
B. Dormancy
C. Respiration
D. Transpiration
Correct Answer:
Totipotency
Explanation:
Totipotency is the ability of a living plant cell to form a complete plant under suitable tissue culture conditions.
4. Which plant hormone is commonly added to tissue culture media to stimulate cell division?
A. Cytokinin
B. Ethylene
C. Abscisic acid
D. Gibberellin
Correct Answer:
Cytokinin
Explanation:
Cytokinins are added to tissue culture media because they stimulate cell division and help in the development of shoots.
5. The production of many genetically similar plants through tissue culture is called:
A. Micropropagation
B. Pollination
C. Fertilization
D. Germination
Correct Answer:
Micropropagation
Explanation:
Micropropagation is a tissue culture technique used to produce numerous genetically similar plants from a small piece of plant tissue.
6. In tissue culture, cytokinins are especially useful for multiplying:
A. Shoots
B. Fruits
C. Seeds
D. Pollen grains
Correct Answer:
Shoots
Explanation:
Cytokinins stimulate cell division and shoot multiplication, making them important in the multiplication stage of tissue culture.
7. Which part of a plant is commonly used as an explant for starting tissue culture?
A. Small piece of plant tissue
B. A mature fruit only
C. A dry seed coat only
D. A piece of soil
Correct Answer:
Small piece of plant tissue
Explanation:
An explant is a small piece of plant tissue placed on a sterile nutrient medium to begin tissue culture.
8. The use of cytokinin in tissue culture can help produce disease-free plants by enabling:
A. Rapid multiplication of selected plant material
B. Formation of soil minerals
C. Destruction of all plant cells
D. Prevention of cell division
Correct Answer:
Rapid multiplication of selected plant material
Explanation:
Tissue culture with suitable cytokinin levels allows rapid multiplication of selected plant tissues, including healthy or disease-free material.
9. In plant tissue culture, an undifferentiated mass of cells is known as:
A. Callus
B. Anther
C. Stoma
D. Fruit
Correct Answer:
Callus
Explanation:
A callus is an unorganized mass of dividing plant cells that can form in tissue culture and later develop into shoots or roots.
10. Cytokinins in tissue culture are important because they primarily stimulate:
A. Cell division
B. Water loss
C. Leaf abscission
D. Seed drying
Correct Answer:
Cell division
Explanation:
Cytokinins promote cell division in cultured plant tissues, supporting callus growth and the formation of new shoots.
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