biology : class12 Recognition Sequences : DNA Isolation, Restriction Enzymes, Vector, Ligation, Host Cell : Recombinant DNA Technology MCQ Questions and Answers
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Recognition Sequences
MCQ questions and answers.
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MCQ Questions
1. What is a recognition sequence in recombinant DNA technology?
A.
A specific DNA sequence recognized by a restriction enzyme
B.
A sequence that codes for a ribosomal protein
C.
A region where DNA replication always begins
D.
A sequence that removes introns from RNA
Correct Answer:
A specific DNA sequence recognized by a restriction enzyme
Explanation:
A recognition sequence is a particular nucleotide sequence at which a restriction enzyme binds and cuts DNA.
2. Which sequence is recognized by the restriction enzyme EcoRI?
A.
AAGCTT
B.
GGATCC
C.
GAATTC
D.
CTGCAG
Correct Answer:
GAATTC
Explanation:
EcoRI recognizes the DNA sequence GAATTC and cuts within this recognition site.
3. Which sequence is recognized by HindIII?
A.
GAATTC
B.
AAGCTT
C.
GGCC
D.
CCC GGG
Correct Answer:
AAGCTT
Explanation:
HindIII specifically recognizes the nucleotide sequence AAGCTT.
4. Which sequence is the recognition site for BamHI?
A.
GGATCC
B.
CTGCAG
C.
AAGCTT
D.
GAATTC
Correct Answer:
GGATCC
Explanation:
BamHI recognizes the specific DNA sequence GGATCC.
5. Restriction enzyme recognition sequences are commonly described as palindromic because they read the same in which direction on the two DNA strands?
A.
Only from 3′ to 5′ on one strand
B.
From 5′ to 3′ on both complementary strands
C.
Only from 5′ to 3′ on one strand
D.
From 3′ to 5′ on both non-complementary strands
Correct Answer:
From 5′ to 3′ on both complementary strands
Explanation:
A palindromic recognition sequence has the same sequence when each complementary strand is read in the 5′ to 3′ direction.
6. Which restriction enzyme recognizes the sequence CTGCAG?
A.
EcoRI
B.
HindIII
C.
PstI
D.
BamHI
Correct Answer:
PstI
Explanation:
PstI recognizes the specific palindromic sequence CTGCAG.
7. What determines which DNA sites a restriction enzyme can recognize?
A.
The specific nucleotide sequence of the site
B.
The length of the host cell
C.
The amount of cellular RNA
D.
The number of ribosomes in the cell
Correct Answer:
The specific nucleotide sequence of the site
Explanation:
Each restriction enzyme recognizes a particular nucleotide sequence in DNA.
8. Which of the following is a palindromic DNA recognition sequence?
A.
GAATTC
B.
GACTTC
C.
ATGGCA
D.
TACGGA
Correct Answer:
GAATTC
Explanation:
GAATTC is a palindromic recognition sequence because its complementary strand has the same 5′ to 3′ sequence.
9. Which enzyme recognizes the sequence CCCGGG?
A.
SmaI
B.
BamHI
C.
HindIII
D.
PstI
Correct Answer:
SmaI
Explanation:
SmaI recognizes the specific DNA sequence CCCGGG.
10. Why are recognition sequences important in recombinant DNA technology?
A.
They identify the sites where restriction enzymes cut DNA
B.
They produce amino acids directly
C.
They prevent DNA from containing nucleotides
D.
They convert DNA into RNA
Correct Answer:
They identify the sites where restriction enzymes cut DNA
Explanation:
Recognition sequences guide restriction enzymes to specific positions in DNA, allowing precise cutting during recombinant DNA work.
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