1. What is the origin of replication in a cloning vector?
A.
The site where DNA replication begins
B.
The site where proteins are destroyed
C.
The region where cell division ends
D.
The site where restriction enzymes are produced
Correct Answer:
The site where DNA replication begins
Explanation:
The origin of replication, or ori, is the specific DNA sequence where replication of the vector DNA starts.
2. What is the main function of the origin of replication in a plasmid vector?
A.
To initiate replication of the plasmid DNA
B.
To break the plasmid DNA into fragments
C.
To produce antibiotics directly
D.
To attach the plasmid to the cell wall
Correct Answer:
To initiate replication of the plasmid DNA
Explanation:
The origin of replication allows the plasmid DNA to begin copying itself inside the host cell.
3. The origin of replication is commonly abbreviated as what?
A.
ori
B.
rRNA
C.
mRNA
D.
tRNA
Correct Answer:
ori
Explanation:
The term ori is the standard abbreviation for the origin of replication in a vector.
4. Why is an origin of replication necessary in a cloning vector?
A.
It enables the vector to replicate inside the host cell
B.
It enables the vector to digest proteins
C.
It enables the vector to form a cell membrane
D.
It enables the vector to produce restriction enzymes
Correct Answer:
It enables the vector to replicate inside the host cell
Explanation:
A functional origin of replication is required so that the vector can make copies of itself in the host cell.
5. A plasmid without a functional origin of replication would most directly be unable to do what?
A.
Replicate inside the host cell
B.
Enter the restriction enzyme
C.
Become a protein
D.
Form a ribosome
Correct Answer:
Replicate inside the host cell
Explanation:
Without a functional ori, the plasmid cannot initiate DNA replication in the host cell.
6. The origin of replication is a feature of which recombinant DNA tool?
A.
Vector
B.
DNA ligase enzyme
C.
Restriction enzyme
D.
Amino acid
Correct Answer:
Vector
Explanation:
A cloning vector contains an origin of replication so that it can replicate after entering a suitable host cell.
7. What does the presence of an origin of replication allow in a host cell?
A.
Multiplication of the vector DNA
B.
Conversion of DNA into lipids
C.
Destruction of all host DNA
D.
Formation of a new cell wall by the vector
Correct Answer:
Multiplication of the vector DNA
Explanation:
The ori permits the vector DNA to be copied, resulting in multiplication of the recombinant DNA molecule.
8. The copy number of a plasmid is related to its what?
A.
Origin of replication
B.
Cell membrane thickness
C.
Protein color
D.
Ribosome size
Correct Answer:
Origin of replication
Explanation:
The origin of replication helps determine how many copies of a plasmid can be maintained in a host cell.
9. Where does replication of a plasmid begin?
A.
At its origin of replication
B.
At its antibiotic molecule
C.
At its cell membrane
D.
At its ribosome
Correct Answer:
At its origin of replication
Explanation:
Plasmid DNA replication starts at the specific sequence called the origin of replication.
10. Which characteristic is important for a vector to be maintained in a host cell?
A.
A functional origin of replication
B.
A permanently damaged DNA sequence
C.
Absence of all DNA sequences
D.
Inability to copy its DNA
Correct Answer:
A functional origin of replication
Explanation:
A functional ori allows the vector to replicate and therefore be maintained in the host cell.
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