First Substrate-Level Phosphorylation MCQ Questions and Answers
1. Which reaction represents the first substrate-level phosphorylation in glycolysis?
A. Glucose is converted into glucose-6-phosphate
B. 1,3-bisphosphoglycerate is converted into 3-phosphoglycerate
C. Phosphoenolpyruvate is converted into pyruvate
D. Fructose-6-phosphate is converted into fructose-1,6-bisphosphate
Correct Answer:
1,3-bisphosphoglycerate is converted into 3-phosphoglycerate
Explanation:
The first substrate-level phosphorylation transfers a phosphate group from 1,3-bisphosphoglycerate to ADP, forming 3-phosphoglycerate and ATP.
2. Which enzyme catalyzes the first substrate-level phosphorylation of glycolysis?
A. Hexokinase
B. Phosphofructokinase
C. Phosphoglycerate kinase
D. Pyruvate kinase
Correct Answer:
Phosphoglycerate kinase
Explanation:
Phosphoglycerate kinase catalyzes the transfer of a phosphate group from 1,3-bisphosphoglycerate to ADP during the first substrate-level phosphorylation.
3. What molecule receives the phosphate group during the first substrate-level phosphorylation?
A. ATP
B. ADP
C. Glucose
D. NAD+
Correct Answer:
ADP
Explanation:
ADP receives the phosphate group from 1,3-bisphosphoglycerate and is converted into ATP.
4. What is the main product formed when 1,3-bisphosphoglycerate donates a phosphate group in glycolysis?
A. Glucose-6-phosphate
B. Fructose-1,6-bisphosphate
C. 3-phosphoglycerate
D. Pyruvate
Correct Answer:
3-phosphoglycerate
Explanation:
When 1,3-bisphosphoglycerate transfers one phosphate group to ADP, it becomes 3-phosphoglycerate.
5. How is ATP formed during the first substrate-level phosphorylation?
A. By direct transfer of a phosphate group to ADP
B. By splitting ATP into ADP and phosphate
C. By removing oxygen from glucose
D. By transferring electrons to NAD+
Correct Answer:
By direct transfer of a phosphate group to ADP
Explanation:
Substrate-level phosphorylation forms ATP through the direct transfer of a phosphate group from a phosphorylated substrate to ADP.
6. Where does the first substrate-level phosphorylation of glycolysis occur?
A. Mitochondrial matrix
B. Inner mitochondrial membrane
C. Cytoplasm
D. Nucleus
Correct Answer:
Cytoplasm
Explanation:
Glycolysis, including the first substrate-level phosphorylation, takes place in the cytoplasm.
7. Which high-energy intermediate donates a phosphate group in the first substrate-level phosphorylation?
A. Glucose-6-phosphate
B. 1,3-bisphosphoglycerate
C. Fructose-6-phosphate
D. Pyruvate
Correct Answer:
1,3-bisphosphoglycerate
Explanation:
1,3-bisphosphoglycerate contains a high-energy phosphate bond that allows it to transfer a phosphate group to ADP.
8. How many ATP molecules are formed from one glucose molecule during the first substrate-level phosphorylation?
A. One ATP molecule
B. Two ATP molecules
C. Three ATP molecules
D. Four ATP molecules
Correct Answer:
Two ATP molecules
Explanation:
Each glucose molecule produces two molecules of 1,3-bisphosphoglycerate, so the first substrate-level phosphorylation forms two ATP molecules.
9. The first substrate-level phosphorylation converts ADP into which molecule?
A. AMP
B. ATP
C. NADH
D. FADH2
Correct Answer:
ATP
Explanation:
A phosphate group is added directly to ADP during this reaction, producing ATP.
10. Which pair correctly describes the first substrate-level phosphorylation in glycolysis?
A. 1,3-bisphosphoglycerate and phosphoglycerate kinase
B. Glucose and hexokinase
C. Phosphoenolpyruvate and pyruvate kinase
D. Fructose-6-phosphate and phosphofructokinase
Correct Answer:
1,3-bisphosphoglycerate and phosphoglycerate kinase
Explanation:
The first substrate-level phosphorylation uses 1,3-bisphosphoglycerate as the phosphate donor and is catalyzed by phosphoglycerate kinase.
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