Fructose-1,6-Bisphosphate Formation MCQ Questions and Answers
1. Which molecule is phosphorylated to form fructose-1,6-bisphosphate during glycolysis?
A. Glucose
B. Fructose-6-phosphate
C. Glyceraldehyde-3-phosphate
D. Pyruvate
Correct Answer:
Fructose-6-phosphate
Explanation:
Fructose-6-phosphate is phosphorylated to form fructose-1,6-bisphosphate.
2. Which enzyme catalyzes the formation of fructose-1,6-bisphosphate?
A. Hexokinase
B. Phosphoglycerate kinase
C. Phosphofructokinase-1
D. Pyruvate kinase
Correct Answer:
Phosphofructokinase-1
Explanation:
Phosphofructokinase-1 catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate.
3. Which molecule provides the phosphate group during fructose-1,6-bisphosphate formation?
A. ATP
B. NADH
C. ADP
D. Pyruvate
Correct Answer:
ATP
Explanation:
ATP donates a phosphate group when fructose-6-phosphate is converted into fructose-1,6-bisphosphate.
4. How many ATP molecules are used in the formation of one molecule of fructose-1,6-bisphosphate?
A. None
B. One
C. Two
D. Four
Correct Answer:
One
Explanation:
One ATP molecule is consumed to add the second phosphate group to fructose-6-phosphate.
5. Fructose-1,6-bisphosphate contains how many phosphate groups?
A. One
B. Two
C. Three
D. Four
Correct Answer:
Two
Explanation:
The molecule has phosphate groups attached at carbon 1 and carbon 6, so it contains two phosphate groups.
6. At which carbon position is the additional phosphate group added to fructose-6-phosphate?
A. Carbon 1
B. Carbon 2
C. Carbon 3
D. Carbon 6
Correct Answer:
Carbon 1
Explanation:
Phosphofructokinase-1 adds a phosphate group at carbon 1 of fructose-6-phosphate, producing fructose-1,6-bisphosphate.
7. Fructose-1,6-bisphosphate formation is which type of reaction in glycolysis?
A. Phosphorylation
B. Dehydration
C. Oxidation
D. Reduction
Correct Answer:
Phosphorylation
Explanation:
The reaction is a phosphorylation because a phosphate group from ATP is added to fructose-6-phosphate.
8. Where does the formation of fructose-1,6-bisphosphate occur in a cell?
A. Nucleus
B. Mitochondrial matrix
C. Cytoplasm
D. Chloroplast thylakoid
Correct Answer:
Cytoplasm
Explanation:
Glycolysis, including fructose-1,6-bisphosphate formation, takes place in the cytoplasm.
9. What is the direct product formed when phosphofructokinase-1 acts on fructose-6-phosphate?
A. Glucose-6-phosphate
B. Fructose-1,6-bisphosphate
C. Glyceraldehyde-3-phosphate
D. Phosphoenolpyruvate
Correct Answer:
Fructose-1,6-bisphosphate
Explanation:
The enzyme phosphofructokinase-1 converts fructose-6-phosphate into fructose-1,6-bisphosphate.
10. The formation of fructose-1,6-bisphosphate represents which ATP-related stage of glycolysis?
A. ATP production
B. ATP consumption
C. NADH production
D. Carbon dioxide release
Correct Answer:
ATP consumption
Explanation:
One ATP is consumed during the phosphorylation of fructose-6-phosphate to form fructose-1,6-bisphosphate.
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